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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 250
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Cell_line_name |
A-204
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Brief_description |
confirmed as human with IEF of AST, MDH established from the rhabdomyosarcoma
of a 1-year-old girl in 1973; reported to form small malignant tumors in nude mice
consistent with embryonic rhabdomyosarcoma
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Description |
human rhabdomyosarcoma established from the rhabdomyosarcoma of a 1-year-old
girl in 1973; reported to form small malignant tumors in nude mice consistent with
embryonic rhabdomyosarcoma confirmed as human with IEF of AST, MDH
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Depositor |
obtained from DKFZ Tumorbank, Heidelberg, Germany
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Morphology |
epithelial-like adherent cells growing in monolayers
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Culture_conditions |
85-90% McCoy's 5A + 10-15% FBS split confluent culture 1 x per week at up to 1:5
using trypsin/EDTA; seed out directly in a 80 cm 2 culture flask, cells will reach
confluency within 5-8 days; cells may clump after trypsinization and may be difficult to
disperse at 37C with 5% CO2 cell harvest of about 5-10 x 106 cells/80 cm 2
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Viruses |
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
HTLV-I/II -, SMRV -
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Properties |
cytokeratin (+), cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 (+), cytokeratin-18 (+),
desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule
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Doubling_time |
about 36 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
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Fingerprint |
multiplex PCR of minisatellite markers revealed a unique DNA profile
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Cytogenetics |
human near diploid karyotype with tetraploid sideline (31%) and 3.5% polyploidy -
46(41-46)<2n>XX - no consistent chromosome abnormality detected
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Bibliographic_reference |
Giard et al., J Natl Cancer Inst, 51: 59 (1973) [PMID: 4357758
] |
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